Cytotoxic effects of Gliotoxin extracted from Candida albicans isolated from patients with urinary tract infection
Abstract
The findings of the toxicity test demonstrated the toxicity of exposure to the gliotoxin produced by candida albicans. Elevated medication levels were associated with an increased risk of blood deterioration. At a concentration of 200 µg/mL, the rate of degradation was 2.21%, and at a value of 100 µg/mL, it was 1.97%. The toxicity increased with prolonged contact with the fungus. And the Samples of gliotoxin were also tested on human lymphocytes to determine their cytotoxic effects. Using the methylthiazol tetrazolium (MTT) bioassay, the cells were subjected to four different doses of gliotoxin (100, 50, 25, and 12.5 µg/mL). Cell growth was shown to be concentration-dependent, with the sample exhibiting growth inhibition percentages at the corresponding concentrations of 33.82%, 10.16%, 5.7%, 0.0%, and 0.0%. The possible DNA damage caused by gliotoxin was evaluated by extracting DNA from lymphocytes and performing electrophoresis on a 1% agarose gel. The findings indicated that gliotoxin has the capacity to destroy or impair DNA. The study established a linear correlation among gliotoxin concentration, cell growth inhibition, and the degree of DNA damage in human lymphocytes. The study investigated the genotoxic effects of Gliotoxin (GT) on human lymphocytes, using single cell electrophoresis and a comet assay. Results showed significant DNA damage in these cells, highlighting GT’s genotoxic impact. The comet assay revealed no significant differences in comet length between the control and concentration 50 µg, but the concentration 100 µg that shown significant changes in head diameter, head, tail, and tail content. These findings highlight GT adverse impact on DNA integrity.
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