Co-stimulation effect of fluid shear stress-material surface chemistry on the behavior of human umbilical vein endothelial cells
Abstract
Objective: The improvement of bone repair scaffolds to enhance their bioactivity and in vivo vascularization is a current research hotspot. Method: HUVECs are subjected to both fluid shear stress (FSS) and chemical stimuli simultaneously. The release of ATP, NO, and the expression of eNOS were examined. The adhesion spots and cytoskeleton formed by HUVEC on the material surface were also observed. Result: LFSS (low fluid shear stress, 5 dyn/cm2) did not trigger a response on Glass and -NH2 HUVECs, but induced a strong response on -OH and -CH3, while PFSS (physiological fluid shear stress, 15 dyn/cm2) and HFSS (high fluid shear stress, 20 dyn/cm2) generated responses of all groups of cells, among which the strongest response level was from the -NH2 group, followed by Glass, and among which equal response levels of the -OH and -CH3 groups existed at the lowest. Conclusion: The chemical functional groups changed the initial threshold of HUVECs response to FSS and the shear force stimulation threshold for optimal cellular response by influencing the quality of adhesion spots and cytoskeleton formed by HUVECs on the surface of the material, thereby altering the response state of endothelial cells to shear force stimulation.
References
1. Steven J, Kunne TB, Malas CM, Semeins A. Comprehensive transcriptome analysis of fluid shear stress altered gene expression in renal epithelial cells. Journal of cellular physiology. 2018.
2. Eric M, Merjem M, David HK. Review on material parameters to enhance bone cell function in vitro and in vivo. Biochem Soc Trans; 2020.
3. Litvina EA, Semenistyy AA. A case report of extensive segmental defect of the humerus treated with Masquelet technique. Journal of Shoulder and Elbow Surgery. 2020; 29(7): 1368-1374. doi: 10.1016/j.jse.2020.03.018
4. Wang C, Huang W, Zhou Y, et al. 3D printing of bone tissue engineering scaffolds. Bioactive Materials. 2020; 5(1): 82-91. doi: 10.1016/j.bioactmat.2020.01.004
5. Colin YH. Dissection of mechanical force in living cells by super-resolved traction force microscopy. Nature protocols erecipes for researchers; 2017.
6. Gong X, Zhao X, Li B, et al. Quantitative Studies of Endothelial Cell Fibronectin and Filamentous Actin (F-Actin) Coalignment in Response to Shear Stress. Microscopy and Microanalysis. 2017; 23(5): 1013-1023. doi: 10.1017/s1431927617012454
7. Li YSJ, Haga JH, Chien S. Molecular basis of the effects of shear stress on vascular endothelial cells. Journal of Biomechanics. 2005; 38(10): 1949-1971. doi: 10.1016/j.jbiomech.2004.09.030
8. Schwartz MA. Integrins and Extracellular Matrix in Mechanotransduction. Cold Spring Harbor Perspectives in Biology. 2010; 2(12): a005066-a005066. doi: 10.1101/cshperspect.a005066
9. Gimbrone MA, García-Cardeña G. Endothelial Cell Dysfunction and the Pathobiology of Atherosclerosis. Circulation Research. 2016; 118(4): 620-636. doi: 10.1161/circresaha.115.306301
10. Seymour RS, Hu Q, Snelling EP. Blood flow rate and wall shear stress in seven major cephalic arteries of humans. Journal of Anatomy. 2019; 236(3): 522-530. doi: 10.1111/joa.13119
11. Harrison D, Griendling KK, Landmesser U, et al. Role of oxidative stress in atherosclerosis. The American journal of cardiology. 2003; 91(3): 7-11. doi: 10.1016/S0002-9149(02)03144-2
12. Zhang Y, Habibovic P. Delivering Mechanical Stimulation to Cells: State of the Art in Materials and Devices Design. Advanced Materials. 2022; 34(32). doi: 10.1002/adma.202110267
13. Castro N, Ribeiro S, Fernandes MM, et al. Physically Active Bioreactors for Tissue Engineering Applications. Advanced Biosystems. 2020; 4(10). doi: 10.1002/adbi.202000125
14. Harburger DS, Calderwood DA. Integrin signalling at a glance. Journal of Cell Science. 2009; 122(2): 159-163. doi: 10.1242/jcs.018093
15. Cao H, Huang D, Guo L, et al. Effects of different chemical groups on behaviors of bladder cancer cells. Journal of Biomedical Materials Research Part A. 2020; 108(12): 2484-2490. doi: 10.1002/jbm.a.36999
16. Shan Y, Yu L, Li Y, et al. Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin. PLoS Biology. 2009; 7(5): e1000116. doi: 10.1371/journal.pbio.1000116
17. Albinsson S, Hellstrand P. Integration of signal pathways for stretch-dependent growth and differentiation in vascular smooth muscle. American Journal of Physiology-Cell Physiology. 2007; 293(2): C772-C782. doi: 10.1152/ajpcell.00622.2006
18. Cui LH, Joo HJ, Kim DH, et al. Manipulation of the response of human endothelial colony-forming cells by focal adhesion assembly using gradient nanopattern plates. Acta Biomaterialia. 2018; 65: 272-282. doi: 10.1016/j.actbio.2017.10.026
19. Hai N, Feng Y, Lu L, et al. Effect of Focal Adhesion Proteins on Endothelial Cell Adhesion, Motility and Orientation Response to Cyclic Strain. Annals of Biomedical Engineering. 2009; 38(1): 208-222. doi: 10.1007/s10439-009-9826-7
20. Ruze A, Zhao Y, Li H, et al. Low shear stress upregulates the expression of fractalkine through the activation of mitogen-activated protein kinases in endothelial cells. Blood Coagulation & Fibrinolysis. 2018; 29(4): 361-368. doi: 10.1097/mbc.0000000000000701
21. Cai D, Chen C, Su Y, et al. LRG1 in pancreatic cancer cells promotes inflammatory factor synthesis and the angiogenesis of HUVECs by activating VEGFR signaling. Journal of Gastrointestinal Oncology. 2022; 13(1): 400-412. doi: 10.21037/jgo-21-910
22. Dar W, Sharon A, Ruth G, et al. Mechanical Compression Effects on the Secretion of vWF and IL-8 by Cultured Human Vein Endothelium. PloS one; 2017.
23. Kohn JC, Zhou DW, Bordeleau F, et al. Cooperative Effects of Matrix Stiffness and Fluid Shear Stress on Endothelial Cell Behavior. Biophysical Journal. 2015; 108(3): 471-478. doi: 10.1016/j.bpj.2014.12.023
24. Li Y, Qin Z, Zhou L, et al. Collective influence of substrate chemistry with physiological fluid shear stress on human umbilical vein endothelial cells. Cell Biology International. 2021; 45(9): 1926-1934. doi: 10.1002/cbin.11632
25. Zhao X, Peng X, Sun S, et al. Role of kinase-independent and -dependent functions of FAK in endothelial cell survival and barrier function during embryonic development. Journal of Cell Biology. 2010; 189(6): 955-965. doi: 10.1083/jcb.200912094
26. Tzima E. Activation of integrins in endothelial cells by fluid shear stress mediates Rho-dependent cytoskeletal alignment. The EMBO Journal. 2001; 20(17): 4639-4647. doi: 10.1093/emboj/20.17.4639
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