Potential biological targets of phytochemicals in regulating inflammatory responses in lung injury

Article ID: 1369

Abstract

Acute lung injury (ALI) is a pulmonary condition caused by various factors, characterized by high mortality rates and significant clinical challenges. The onset of lung injury is closely associated with excessive inflammatory responses, making the modulation of these responses a critical target for ALI therapy. Despite the growing interest in phytochemicals, the mechanisms through which they modulate inflammatory pathways in lung injury remain poorly understood. This knowledge gap underscores the need for further investigation into the biological targets of phytochemicals in regulating lung injury-induced inflammation. The purpose of this study is to identify specific phytochemical compounds that can mitigate inflammation by targeting key signaling pathways, particularly by inhibiting Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB); p38 Mitogen-Activated Protein Kinase (p38 MAPK); and c-Jun N-terminal Kinase (JNK) pathways, thereby reducing pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β. A mouse model of acute lung injury was established, and different concentrations of phytochemicals were administered. Combined with biochemical analyses, immunohistochemical staining, and Western blot experiments, the inhibitory effects of phytochemicals on lung injury and related inflammatory cytokines were evaluated. The results indicate that phytochemicals can significantly suppress the inflammatory response caused by lung injury, reducing the levels of inflammatory cytokines such as TNF-α, IL-6, and IL-1β (p < 0.01). Pathological analyses revealed substantial improvement in lung tissue, particularly in the high-concentration intervention group. Western blot results demonstrated that phytochemicals exert anti-inflammatory effects by inhibiting the activation of NF-κB, p38 MAPK, and JNK signaling pathways. This study provides novel insights into the potential molecular mechanisms underlying the anti-inflammatory effects of phytochemicals in ALI, and identifies specific biological targets for their clinical application, highlighting their promising therapeutic potential.